an experimental study on broiler chicken co-infected with the specimens containing avian influenza (h9 subtype) and infectious bronchitis (4/91 strain) viruses

Authors

s. seifi

k. asasi

a. mohammadi

abstract

this experimental study was carried out to induce a co-infection of h9 aiv and ibv with inoculumsprepared from tracheal scrubbed epithelium tissues in natural co-infected birds to rule out the effect of fieldundesired environmental conditions and also other infectious causative agents. eighty 1-day-old broilerswere randomly divided into four equal groups. at 21 days of age, three test groups were inoculatedintranasally with prepared inoculums containing ibv and h9 aiv alone and a co-infected group. the fourthgroup remained uninfected as a control group. the results showed that experimental co-infection of aiv andibv increased the severity of clinical signs, mortality rate and gross lesions. the hi titer against aivinfection in the co-infected group was significantly higher than the hi titer of other groups, which mayindicate that ibv could promote the propagation of h9n2 aiv or stimulate the immune response.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

An experimental study on broiler chicken co-infected with the specimens containing avian influenza (H9 subtype) and infectious bronchitis (4/91 strain) viruses

This experimental study was carried out to induce a co-infection of H9 AIV and IBV with inoculumsprepared from tracheal scrubbed epithelium tissues in natural co-infected birds to rule out the effect of fieldundesired environmental conditions and also other infectious causative agents. Eighty 1-day-old broilerswere randomly divided into four equal groups. At 21 days of age, three test groups we...

full text

Chinese and Global Distribution of H9 Subtype Avian Influenza Viruses

H9 subtype avian influenza viruses (AIVs) are of significance in poultry and public health, but epidemiological studies about the viruses are scarce. In this study, phylogenetic relationships of the viruses were analyzed based on 1233 previously reported sequences and 745 novel sequences of the viral hemagglutinin gene. The novel sequences were obtained through large-scale surveys conducted in ...

full text

Detection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

full text

Development of an immunochromatographic strip for rapid detection of H9 subtype avian influenza viruses.

An immunochromatographic strip was developed for the detection of the H9 subtype of avian influenza viruses (H9AIVs) in poultry, using two monoclonal antibodies (MAb), 4C4 for H9AIV hemagglutinin (HA) and 4D4 for nucleoprotein. The 4C4 MAb was labeled with colloidal gold as the detection reagent, and the 4D4 MAb was blotted on the test line while a goat anti-mouse antibody was used on the contr...

full text

Genotyping of Infectious bronchitis viruses isolated from broiler chicken farms in Iran during 2015-2016

BACKGROUND: Avian infectious bronchitis is considered as an important viral disease worldwide. Genotyping based on the S1 subunit of spike protein gene of the causative agent, avian infectious bronchitis virus, can be used to classify IBV isolates. Objective: This survey was carried out to characterize the infectious bronchitis virus (IBV) genotypes circulating in Iran and determine their preva...

full text

Prevalence of avian infectious bronchitis virus in broiler chicken farms in south of Iraq, 2014 – 2015

Avian infectious bronchitis (IB), caused by a gammacoronavirus, is an OIE-listed (List B) disease and characterized by respiratory and renal involvements, causing high mortality, and economic loss in both layers and broilers. In comparison with other diagnostic methods, real-time polymerase chain reaction (RT-PCR) and conventional RT-PCR are potent, more sensitive and faster techniques for infe...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of veterinary research

Publisher: shiraz university

ISSN 1728-1997

volume 13

issue 2 2012

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023